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Effects of Intragastric Quinine, Alone or Combined With L-leucine, on Postprandial Glycaemic Control
In this study, participants will receive, in a randomized, double-blind fashion, an intragastric bolus administration of either (i) 300 mg quinine, (ii) 5 g L-leucine, (iii) a combination of (i)+(ii), or (iv) control, before 350 ml (500 kcal) of a mixed-nutrient drink, to evaluate the effects on postprandial blood glucose, gastric emptying, and the hormone responses to the mixed-nutrient drink. Study visits will be separated by 3-7 days and participants will receive one treatment per visit. On each study visit, the participant will be intubated with a nasogastric feeding tube.
At t= - 60 min (08:30 am), a baseline blood sample, visual analogue scale questionnaire (VAS), and breath sample will be collected and quinine or control will be administered through the feeding tube. 30 min later (at t= - 30 min), L-leucine or control will be administered over 2 min after which the feeding tube will be removed immediately. At t = -45, -30, -15, and -1 min further blood samples will be collected and VAS completed.
At t = -1 min, participants will consume, within 1 minute, a mixed-nutrient drink, labelled with 100 mg of 1-13C-acetate for measurement of gastric emptying by breath sampling. Blood samples, VAS, and breath samples will be taken at regular intervals between t = 0-180 min.
Study details:
This trial aims to assess the effects of intragastric administration of quinine, combined with L-leucine, on postprandial blood glucose, gastric emptying, gut and gluco-regulatory hormones, as well as GI symptoms in response to a mixed-nutrient drink. Each participant will be studied on 4 occasions, separated by 3-7 days. Participants will receive, in randomized, double-blind fashion, an intragastric bolus of (i) 300 mg quinine, (ii) 5 g L-leucine, (iii) combination of (i)+(ii), or (iv) control.
Due to the low water solubility, L- leucine will be provided as a suspension using 5 ml of 'the suspending agent' Ora-Plus (manufactured by Perrigo, Minneapolis). Visits will be carried out at the Clinical Research Facility, Adelaide Medical School, University of Adelaide, by staff and students trained in the required clinical research techniques. Participants will consume a standardized dinner meal (400g McCain's beef lasagne) the night before each study visit by no later than 7 pm.
After fasting for 13. 5 hours overnight and refraining from alcohol and exercise for 24 hours, participants will arrive at the Clinical Research Facility by 8:30 am. Upon arrival, participants will be intubated with a nasogastric, custom-built soft silicon feeding tube (outer diameter: 4mm; Dentsleeve, Mississauga, Ontario, Canada) that will be inserted through an anaesthetized nostril and placed in the stomach.
An intravenous cannula will be placed into a right forearm vein for regular blood sampling. At t = -60 min, a venous baseline blood sample (6 ml) will be collected and the participant will complete a visual analogue scale questionnaire (VAS) to assess GI symptoms (nausea and bloating). Immediately thereafter, the participant will receive a 10- ml intragastric bolus of either 300 mg quinine-hydrochloride (Q-HCl) or water (control), and 30 min later, at t -30 min, an intragastric bolus of L-leucine (100 ml suspension consisting of 5 g L- leucine, 5 ml Ora-Plus and 90 ml 0.
9% saline) or control (5 ml Ora-Plus and 95 ml saline) over 1 min after which time the catheter will be removed immediately. At t = -1 min, the participant will consume, within 1 min, a mixed-nutrient drink (Nestle, 500 kcal, 350 ml, 56 g carbohydrates) labelled with 100 mg of 1-13C-acetate for measurement of gastric emptying by breath sampling. Breath samples will be collected in sealed breath bags at baseline (prior to quinine administration) and at regular intervals between t = 0-180 min, for subsequent analysis of 13CO2 concentration in exhaled breath.
Blood samples for the measurement of glucose and plasma concentrations of hormones will be taken regularly (12 sampling time points in total), and participant complete VAS questionnaires. At t = 180 min, after final blood and breath samples and VAS measurements, the intravenous cannula will be removed and the participant will be served a light lunch, after which they will be allowed to leave the laboratory.
Eligibility criteria
Researchers look for people who fit a certain description, called eligibility criteria. See if you qualify.
Inclusion criteria
Exclusion criteria
Eligibility
Age eligible for study : 18 and older
Healthy volunteers accepted : Yes
Gender eligible for study: Male
Things to know
Study dates
Study start: 2023-02-02
Primary completion: 2024-08-01
Study completion finish: 2024-08-01
Study type
OTHER
Phase
NA
Trial ID
NCT05720390
Intervention or treatment
OTHER: Quinine
OTHER: L-leucine
OTHER: Combination of quinine and L-leucine
OTHER: Control
Conditions
- • Healthy
Find a site
Closest Location:
Clinical Research Facility, Adelaide Health and Medical Sciences Building
Research sites nearby
Select from list below to view details:
Clinical Research Facility, Adelaide Health and Medical Sciences Building
Adelaide, South Australia, Australia
Study Plan
This section provides details of the study plan, including how the study is designed and what the study is measuring.
How is the study designed?
Participant Group/Arm | Intervention/Treatment |
---|---|
ACTIVE_COMPARATOR: Quinine only
| OTHER: Quinine
|
ACTIVE_COMPARATOR: L-leucine only
| OTHER: L-leucine
|
ACTIVE_COMPARATOR: Quinine + L-leucine
| OTHER: Combination of quinine and L-leucine
|
PLACEBO_COMPARATOR: Control
| OTHER: Control
|
What is the study measuring?
Primary outcome
Primary Outcome Measure | Primary Outcome Description | Primary Outcome Time Frame |
---|---|---|
Change from baseline plasma glucose concentration after a mixed-nutrient drink for 3 hours | Plasma glucose concentrations (mmol/L) will be assessed using glucose oxidase method | Blood samples will be taken repeatedly within each study visit (i.e. at baseline (t= 0 minute), after administration of study treatments (t= -45, -30, -15 minutes) and after a mixed-nutrient drink (t= 0, 15, 30, 45, 60, 90, 120 and 180 minutes). |
Secondary outcome
Secondary Outcome Measure | Secondary Outcome Description | Secondary Outcome Time Frame |
---|---|---|
Gastric emptying of a mixed nutrient drink | Measurement of 13CO2 in breath samples, expressed as percentage of 13CO2 recovery per hour | Breath samples will be taken repeatedly on each study visit (i.e. t= 0 (baseline), 5, 10, 15, 20, 25, 30, 35, 40, 45, 50, 55, 60, 75, 90, 105,120, 135, 150, 165, 180 minutes) to construct a gastric emptying profile on each day. |
Plasma concentration of insulin after a mixed-nutrient drink | Plasma insulin concentrations (mU/L) will be assessed using an ELISA immunoassay. | Blood samples will be taken repeatedly within each study visit (i.e. at baseline (t= 0 minute), after administration of study treatments (t= -45, -30, -15 minutes) and after a mixed-nutrient drink (t= 0, 15, 30, 45, 60, 90, 120 and 180 minutes). |
Plasma concentration of glucagon after a mixed-nutrient drink | Plasma glucagon concentrations (pg/mL) will be measured by radioimmunoassay | Blood samples will be taken repeatedly within each study visit (i.e. at baseline (t= 0 minute), after administration of study treatments (t= -45, -30, -15 minutes) and after a mixed-nutrient drink (t= 0, 15, 30, 45, 60, 90, 120 and 180 minutes). |
Plasma concentration of glucagon-like peptide-1 (GLP-1) after a mixed-nutrient drink | Plasma total GLP-1 concentrations (pmol/L) will be measured using a radioimmunoassay | Blood samples will be taken repeatedly within each study visit (i.e. at baseline (t= 0 minute), after administration of study treatments (t= -45, -30, -15 minute) and after a mixed-nutrient drink (t= 0, 15, 30, 45, 60, 90, 120 and 180 minutes). |
Gastrointestinal symptoms (nausea and bloating) | Gastrointestinal symptoms will be measured using a 100-mm Visual Analogue Scale (VAS) questionnaire. The minimum value means no feeling at all, and the highest value means feeling very much. | Visual Analogue ratings will be collected repeatedly within each study visit (i.e. at baseline (t = 0 minute), after administration of treatments (t= -45, -30, -15 minutes) and after mixed-nutrient drink (t= 0, 15, 30, 45, 60, 90, 120 and 180 minutes). |
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